Title : Qualitative phytochemical analysis of the different tissues of Barleria dinteri for possible underground part substitution with aerial parts towards sustainable medicinal plants usage
Abstract:
The objective of the study was to undertake qualitative phytochemical analysis of the different tissues of Barleria dinteri, a traditional herb used against several diseases, for possible plant tissue substitution towards sustainable medicinal plants usage. The different tissues of B. dinteri were extracted using cold maceration method through a serial exhaustive extraction procedure. The extracts were subjected to qualitative phytochemical analysis through chemical screening, thin layer chromatography and UV-visible spectrophotometric analysis. Qualitative phytochemical analysis through chemical screening showed mostly similarities in the phytochemical compositions of the different plant parts with only few differences observed. Anthraquinones were not detected in the extracts of all different plant tissues. The TLC profiles of samples, depicted through the Rf values of resolved compound bands, also showed mostly similarities with few differences. The UV-visible spectrum of the samples showed mostly similar maximum absorbance wavelengths amongst similar extracts of the different plant tissues. From the results of this study, it can be concluded that the qualitative phytochemical profiles of branches, flowers, leaves and roots of B. dinteri are mostly similar. As such, the aerial parts of B. dinteri could be recommended to substitute the usage of the roots in traditional medicine as a contribution to sustainable usage of the plant species.