Title : Prevention of Insulin resistance by herbal compound from fruit-pulp of Eugenia jambolana in fructose fed rats.
Abstract:
Aim: This study aimed to investigate the preventive effect of FIIc, an active principle isolated from Eugenia jambolana, on the development of insulin resistance induced by fructose in rats.
Methodology: Crude aqueous extract of Eugenia jambolana fruit-pulp was purified using Ion exchange column chromatography, yielding FII, which was further purified to obtain FIIc. The purity of FIIc was confirmed by HPLC. FIIc was administered orally at a dose of 15 mg/kg body weight for 60 days to experimental rats. Body weight, blood glucose, serum triglycerides, total cholesterol, LDL-C, HDL-C, liver and muscle glycogen levels, TNF α, serum insulin, insulin resistance, and the insulinogenic index were measured every 30 days up to 60 days.
Results: Fructose feeding for 60 days significantly (p<0.001) increased serum biochemical parameters and decreased liver and skeletal muscle glycogen levels in the untreated control group. Administration of FIIc significantly (p<0.001) reduced blood glucose compared to the untreated control. Body weight, lipid profile, and liver and skeletal muscle glycogen levels were significantly (p<0.001) improved after FIIc treatment. Levels of serum TNF-α and insulin were nearly normalized following FIIc treatment. Insulin resistance and the insulinogenic index significantly improved compared to the untreated control.
Conclusion: Our study demonstrated that FIIc treatment significantly reduces fructose-induced insulin resistance.
