Anti-inflammatory effect and mechanism of citri reticulatae viride pericarpium Water extract

Objectives: This study was designed to examine anti-inflammatory effect and mechanism  of Citri Reticulatae Viride Pericarpium water extract (CRE).
Methods: Cell cytotoxicity was tested with RAW 264.7cells. To investigate anti-inflammatory effectof CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitricoxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). Inaddition, mitrogen-activated protein kinase (MAPK) and nuclear facto rkappaB (NF-κB) were examined by western blottingin LPS-induced RAW 264.7 cell with treated CRE.
Results: In cyto toxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a re sult of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway.
Conclusions: CRE might have anti-inflammatory effect in LPS-induced macrophages  by promoting the production of IL-10.

Audience Take Away:

• If you are reading this paper, you can use green bark as an anti-inflammatory analgesic.
• Anti-inflammatory drugs are commonly used to relieve symptoms such as pain and fever that occur in inflammatory diseases. Among them, non-steroidal anti-inflammatory drugs (NSAIDs) have been reported to have side effects on the digestive system, cardiovascular system, and renal urinary tract. In addition, steroids are also problematic because when used in high doses or for long periods of time, side effects such as drug resistance and cardiovascular and endocrine problems occur. Green bark is a natural product that can be expected to have anti-inflammatory effects without side effects.
• If the active ingredients of blueberry with anti-inflammatory activity are identified and follow-up studies are conducted on the mechanism, I think there will be potential for use in inflammatory diseases associated with IL-10 expression.