Anti rheumatic and analgesic effects by the parent tuberous roots of Aconitum jaluense in adjuvant induced arthritis rats

Title : Anti rheumatic and analgesic effects by the parent tuberous roots of Aconitum jaluense in adjuvant induced arthritis rats

Abstract:

Aim of the study: The aim of this study was to test the anti-rheumatic effects of A. jaluense tubers in acute and chronic arthritis rats, and to assign its ingredients through UHPLC-TOF/MS.

Materials and methods: Subcutaneous injection of carrageenan for acute arthritis and complete Freund’s adjuvant (CFA) for chronic arthritis was carried out in the hind paw of SD rats. The paw volume was measured by a plethysmometer; thermal hyperalgesia was tested using a thermal plantar tester, and mechanical hyperalgesia was evaluated by ankle flexion evoked vocalizations. The expression of c-Fos in the brain hippocampus was measured with the avidin-biotin-peroxidase technique. The ingredients were assigned by UHPLC-TOF/MS, chromatography was performed by UHPLC system with DAD detector and BEH C₁₈ column, and spectroscopy was conducted by ESI-MS system.

Results and discussion: The 80% ethanoic extract of A. jaluense tubers showed an acute anti-inflammatory effect by suppressing the edema volume in the hind paw of carrageenan-stimulated rats. In addition, A. jaluense tubers exerted an anti-rheumatic activity by reducing the secondary swelling volume from an immunological reaction in the left hind paw of CFA-induced chronic arthritis rats. Additionally, oral treatment with the 80% ethanoic extract -showed potent analgesic effects in the arthritis rats by recovering the paw withdrawal latency stimulated by the thermal hyperalgesia and by reducing the vocalization scores evoked by ankle flexion on both hind paws. Moreover, its treatment also indicated an anti-psychiatric effect by controlling the c-Fos protein expression of the brain hippocampus in CFA-stimulated arthritis rats. These results suggested that these therapeutic effects were exhibited by less toxic mono-esterified diterpenoid alkaloids (MDAs), and nontoxic non-esterified diterpenoid alkaloids (NDAs).

Conclusion: A. jaluense tubers may act as viable therapeutic or preventive candidates for acute and chronic arthritis, particularly, for immune-inflammatory rheumatoid arthritis to suppress the pain and psychiatric condition.

Biography:

Dr. Lee studied Pharmaceutical Science at the Ducksung Women’s University, Korea, and graduated as MS in 1988. She then joined the research group of Prof. Kim at the Institute of Pharmaceutical Science, College of Pharmacy Seoul National University. She received her Ph.D. degree in 1998 at the same institution. After one and a half years, postdoctoral fellowship supervised by Dr. Kim at the Institute of Pharmaceutical Science, College of Pharmacy Seoul National University. she joined the Research Institute of Natural Medicine, Toyama, Japan as a postdoctoral researcher supervised by Prof. Hattori, and joined Yonsei University, Kyunghee University, and Seoul National University as a Research Professor. She has published more than 27 research articles in SCI(E) journals.