HYBRID EVENT: You can participate in person at Paris, France or Virtually from your home or work.

8th Edition of International Conference on

Traditional Medicine, Ethnomedicine and Natural Therapies

June 05-07, 2025 | Rome, Italy

Traditional Medicine 2022

Anti inflammation and gene expression profiling of human hair follicle Dermal papilla cells in response to Oryza Sativa Cv Khao Dawk Mali 105 Bran extract

Speaker at Traditional Medicine, Ethnomedicine and Natural Therapies 2022 - Chiranan Khantham
Chiang Mai University, Thailand
Title : Anti inflammation and gene expression profiling of human hair follicle Dermal papilla cells in response to Oryza Sativa Cv Khao Dawk Mali 105 Bran extract


Androgenetic alopecia (AGA) is a multi-factorial hair loss that affects patients’ psychological welfare and social interaction. Currently, synthetic medicines for AGA contain several side effects and narrow mechanisms. Natural alternative medicines have gained attention due to their multi-mode mechanisms and fewer side effects. The enormous amounts of rice bran, an agricultural waste, could cause severe environmental pollution in Thailand. Moreover, rice bran was used for the treatment of cancer, inflammatory diseases, diabetes, and skin diseases in recipes of Thai folk medicine. Therefore, rice bran extract of local rice cultivar, Khao Dawk Mali 105 (KDML105), was assessed for its valuable utilization for AGA treatment regarding anti-inflammatory activity and gene expression regulation related to AGA. The bioactive compound estimation of phenolic, flavonoid, polysaccharide, and protein contents was determined. Griess reaction assay was used to evaluate the anti-inflammatory effect of KDML105 extract in RAW 264.7 murine macrophages. Semiquantitative reverse transcription-polymerase chain reaction analysis was performed to measure the expression level of genes associated with hair growth promotion in human hair follicle dermal papilla cells (hHFDPCs). The result showed that flavonoids were predominantly found in KDML105 extract (48.18 ± 4.90 mg EGCG equivalent/g extract). Nitric oxide production was notably attenuated by KDML105 extract, contributing to anti-inflammatory activity. Furthermore, KDML105 extract increased the mRNA expression of multiple genes associated with hair growth-promoting effects, including SHH, SMO, GIL1, CTNNB1, and VEGF in hHFDPCs. The study provided the evidence to verify that KDML105 extract possesses the abilities to promote hair growth by reducing nitric oxide production and enhancing the gene expressions in the sonic hedgehog, WNT/β-catenin, and VEGF pathways. Thus, KDML105 extract could be a candidate for further studies on the utilization for AGA treatment.


Chiranan Khantham is a PhD candidate under Assistant Professor Dr. Warintorn Ruksiriwanich at the Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Thailand. She holds the degree of Doctor of Pharmacy and Pharmacy Practice Licence in Thailand. She has recently focused on traditional and alternative medicines for hair loss and the candidate gene study of androgenetic alopecia in Thai population. Her research project is supported by National Research Council of Thailand (NRCT): NRCT5-RRI63004-P05 and partially supported by Chiang Mai University. The rice bran samples in her project were obtained from Saleekam Trading Co., Ltd. She also conducts some parts of research at Centro de Investigación Biomédica (CIBM), University of Granada, Spain. Chiranan has published 8 articles in different peer-reviewed journals during her PhD.