HYBRID EVENT: You can participate in person at Tokyo, Japan or Virtually from your home or work.

5th Edition of International Conference on

Traditional Medicine, Ethnomedicine and Natural Therapies

May 22-23, 2023 | Tokyo, Japan

Traditional Medicine 2022

Sayak Ghosh

Speaker at Traditional Medicine, Ethnomedicine and Natural Therapies 2022 - Sayak Ghosh
The Calcutta Homoeopathic Medical College and Hospital, India
Title : Preventive action of Ultra-diluted Staphylococcinum potency gradients against Panton valentine toxin mediated leukocyte destruction

Abstract:

Background: Bacterial resistance has taken a high rise in the past decade, making it a global public health concern. Reports showing a surge in methicillin-resistant Staphylococcus aureus (MRSA) infection among the world population without any effective approaches. Virulence of the pathogen depends on various toxins and enzymes produced by it, among them the bi-component toxin, also known as Panton-Valentine Leukocidn (PVL) hampers the morphological and functional changes in the leukocytes. Staphylococcin is a colicin-type chemical secreted by Staphylococcus aureus helps to prevent growth of organisms other than its progenitor. In this study, we evaluated the efficacy of homoeopathic medicine Staphylococcinum against staphylococcal PVT at different potencies (6CH, 12CH, 30CH and 200CH).

 

Methods: Different potencies of Staphylococcinum were administered in a leucocyte buffy coat preparation infected with staphylococcal suspension (0.5 McFarland’s standard) along with control with alcohol, as alcohol was the vehicle of homeopathic dilutions. They were kept in incubator for 2 hours and then centrifuged at 1200 rpm for 5 minutes. Smears prepared on slides with centrifuged deposits stained by Preston and Morrell’s modified Gram’s method of staining and evaluated under the microscope. For observation under scanning electron microscope (SEM) we added 20μL of each dilution of Staphylococcinum (6CH, 12CH, 30CH, 200CH) in 20μL buffy-coat suspension infected with S. aureus along with one alcohol control (20 μL). Afterwards slides were made and kept for 30 min at room temperature in containing 4% (v/v) glutaraldehyde in distilled water for fixing. Samples were fixed again with cacodylate buffer containing 2.5% (v/v) glutaraldehyde and kept overnight before a final wash with ethanolic concentrations. Samples were finally mounted on an aluminium stub with double-sided carbon tape before observing under SEM.

 

Results: It was observed under light microscopy, that there was extensive destruction of leukocytes in control and 6CH potency, while the degree of destruction decreased markedly from 12CH to 200CH. At 200CH potency, leukocytes were almost normal. The mean percentages of intact leucocytes were 0.73, 0.93, 10.00, 27.67 and 65.00 in control, 6CH, 12CH, 30CH and 200CH potencies, respectively. The findings under SEM showed similar results; massive destruction of leucocytes by S. Aureus in alcohol control, while there was significant destruction of leucocytes with Staphylococcinum 6CH and 12CH. Staphylococcinum 30CH and 200CH showed excellent result as they prevented destruction of leukocyte significantly.

Conclusion: Staphylococcinum higher dilutions (30CH, 200CH) showed a significant anti-staphylococcal property by preventing the leucocyte morphology as seen in light microscopy and SEM. Further study is required to know the biomolecular basis of this effect.

What will audience learn from your presentation?

  • Taking the homoeopathic medicine from clinics to laboratory. The effect of ultra-dilutions in the in vitro model in fundamental research are shown and connection between the modern-day theories are made.
  • In the age of Anti-microbial resistance this medicine can open a new domain in Staphylococcal infection as adjuvants.

Modern day homoeopathic research can get a new direction and dose variations of ultra-dilutions can be established

Biography:

Dr. Sayak Ghosh, is currently working as a Post Graduate Trainee in oldest homoeopathic medical college of Asia, The Calcutta Homoeopathic Medical College and Hospital in India. After a bright academic carrier in undergraduate, he was associated with Dr. Satadal Das, a renowned pathologist and Microbiologist. He gained interest in preclinical research taking the homoeopathic dilutions, while working with him. Till date he has been associated with many research works and he has six published research papers, including fundamental researches in bacteriology, allergy in relation to Homoeopathy. He is currently working on efficacy of homoeopathic medicines in Sars Cov-2 Cytokine storm

Watsapp