Title : Evaluation of Syzygium cumini L seeds extracts for vitamin C protective effect as teratogenic potential in rats
Objective: All parts of Syzygium cumini L are being widely used as herbal remedies in various ailments. Herbal remedies may cause deleterious effects in pregnant women, which may lead to teratogenicity in the fetuses. This study investigated the possible teratogenic effects of S. cumini seeds at three dose levels (250, 500, and 1000 mg/kg orally) according to OECD 414 test guidelines. This study further investigated the protective effect of vitamin C against highest dose of S. cumini and misoprostol (T.S).
Method: One hundred twelve adult albino Wister female rats were divided into 7 groups (n=16). Doses were started from 5th -16th days of gestation. Dams were weighed on 1st, 7th, 14th and 20th days of gestation. Fetuses were delivered by C-section on the 20th day and live, dead or resorbed fetuses were counted, their fetal weight, length and placental weight were measured.
Results: Results showed decrease in weight of 500 mg/kg, misoprostol plus vitamin C group and drastic weight loss and deaths were observed in 1000 mg/kg of S. cumini. Hematoma in fetuses was also observed. Fetal skeletal staining was done using alizarin red and alcian blue. Fetal skeletal abnormalities included unossified bones of fore and hind paw, non-ossified ribs and sternum, unossified regions of the cervical and thoracic regions, and also dumbbell and transversely fused vertebrae.
Conclusion: On the basis of these results, it may be concluded that the use of S. cumini during pregnancy for the treatment of various chronic ailments is not safe. So, it is strongly recommended to avoid its use in pregnancy.